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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in several tumors, which results in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital job in advertising and marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been reported but in-depth nucleotide sequence details is not really however out there.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly incorporates the epitope acknowledged with the antibody.
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This guidebook outlines further probable optimization of mobile and immunoassay parameters to ensure the best possible final results are...
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This guideline outlines more probable optimization of cellular and immunoassay parameters to guarantee the absolute best success are...
Antibodies certain to the blocking peptide now not bind towards the epitope over the focus on protein. By evaluating the staining from your blocked antibody vs . the antibody alone, you can see which staining is distinct.
Focus on Facts Cyclin E belongs on the very conserved cyclin loved ones, whose users are characterised by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Different cyclins show distinctive expression and degradation designs which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E types a complex with and capabilities to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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